Home  ›  analysis of a cell shows that cdc20 is bound to apc. what else should be true?

analysis of a cell shows that cdc20 is bound to apc. what else should be true?

Written By Thursday 7 April 2022 Add Comment Edit
  • Research
  • Open Access
  • Published:

Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

  • Rebecca Lewandowski2,
  • Michael J Mallory  nAff3,
  • Randy Strich2 &
  • Katrina F Cooper2

Cell Division volume viii, Article number:ix (2013) Cite this article

  • 4558 Accesses

  • 5 Citations

  • Metrics details

Abstruse

Background

The execution of meiotic nuclear divisions in S. cerevisiae is regulated by poly peptide deposition mediated by the anaphase promoting circuitous/cyclosome (APC/C) ubiquitin ligase. The correct timing of APC/C activity is essential for normal chromosome segregation. During meiosis, the APC/C is activated by the clan of either Cdc20p or the meiosis-specific cistron Ama1p. Both Ama1p and Cdc20p are targeted for deposition as cells exit meiosis II with Cdc20p being destroyed past APC/CAma1. In this study we investigated how Ama1p is downwardly regulated at the completion of meiosis.

Findings

Hither we show that Ama1p is a substrate of APC/CCdc20 merely non APC/CCdh1 in meiotic cells. Cdc20p binds Ama1p in vivo and APC/CCdc20 ubiquitylates Ama1p in vitro. Ama1p ubiquitylation requires one of two deposition motifs, a D-box and a "KEN-box" like motif called GxEN. Finally, Ama1p degradation does not require its association with the APC/C via its conserved APC/C binding motifs (C-box and IR) and occurs simultaneously with APC/CAma1-mediated Cdc20p degradation.

Conclusions

Dissimilar the cyclical nature of mitotic cell division, meiosis is a linear pathway leading to the production of quiescent spores. This raises the question of how the APC/C is reset prior to spore germination. This and a previous study revealed that Cdc20p and Ama1p direct each others degradation via APC/C-dependent degradation. These findings suggest a model that the APC/C is inactivated by mutual degradation of the activators. In addition, these results support a model in which Ama1p and Cdc20p relocate to the substrate address inside the APC/C cavity prior to degradation.

Background

Meiosis is a specialized developmental program during which diploid nuclei undergo two sequent meiotic divisions to produce haploid gametes. In the budding yeast, spore wall associates follows the second meiotic nuclear partition producing four haploid spores encased in a protective ascus [one]. Like to differentiation programs in higher eukaryotes, meiotic progression is regulated past the transient expression of genes that are either meiosis specific or expressed during both meiotic and mitotic divisions (reviewed in [2]). In addition, progression through the meiotic divisions is also driven by the degradation of key regulatory proteins directed past the highly conserved multi-complex ubiquitin ligase chosen the anaphase promoting complex/cyclosome (APC/C) (reviewed in [3–6]).

During meiosis, the APC/C is sequentially activated by two of the three known Trp-Asp activator (WD40) proteins, Cdc20p (reviewed in [seven, eight]), and Ama1p, the latter of which is only expressed during meiosis [nine, x]. The Cdc20p activated APC/C (written APC/CCdc20) mediates the degradation of several key regulatory proteins including Pds1p (securin) and the Southward-phase cyclin Clb5 during both meiosis I (MI) and meiosis Two (MII) [eight, 11]. Ama1p directs the ubiquitylation of the B-type cyclin Clb1p [10], Cdc20p [12] plus other unknown substrates [13] and co-ordinates exit from MII [12]. APC/CAma1 also activates Smk1p, the meiotic MAP kinase required for spore wall morphogenesis [14] and is required for the early stages of spore wall associates [11, xiii, xv]. The tertiary APC/C activator Cdh1p, is non required for normal meiosis [sixteen].

It has been well documented that APC/C activator proteins recognize substrates through two conserved degrons called the "Destruction-box" (D-box, DB) and "KEN box" that demark the WD40 domain in the activator [17, eighteen]. In improver, Doc1p (Apc10), a conserved component of the APC/C complex, too recognizes these degrons. These findings have lead to the model that substrates are recruited to the APC/C by bounden to a bipartite substrate receptor composed of an activator poly peptide and Doc1p ([xix] and reviewed in [xx]). During meiosis, Ama1p recognizes the D-box besides as variant of the KEN box called GxEN [ten, 12] whereas Cdc20p recognizes the D-box and the KEN box [21, 22]. However, in Xenopus egg extracts the APC/C recognizes destruction motifs directly, in both a Cdc20p and Cdh1p-independent manner [23]. Similarly, much is known about how the activator proteins bind to the APC/C [five]. Structural analysis of Cdh1p has shown that a domain called the C-box interacts with Apc2p [24]. Some other domain termed the IR motif promotes the clan of the activator with the TPR region of several APC/C subunits (Cdc16p, Cdc23p and Cdc27p) [25–28]. Doc1p (Apc10p), a subunit of the APC/C, also assembly with the TPR subunits via its IR tail [29, xxx]. During meiosis, both the C-box and IR domains are required for Ama1p and Cdc20p office [12]. Nonetheless, mutational analysis revealed that the C-box in Ama1p is significantly more than important for meiotic progression than the IR motif [12]. Similarly, during mitotic cell division, the IR box of Cdc20p is not required for function but contributes to APC/C dependent turnover [three, six].

Although much is known almost how the APC/C is activated during meiotic divisions (reviewed in [8]), considerably less is known well-nigh how this ligase is inactivated every bit cells consummate meiotic program. This is an important question as APC/C inactivation at the end of meiosis may be critical to permit the spore to reenter the mitotic jail cell cycle. Our previous studies have shown that both Ama1p and Cdc20p are down regulated as cells exit from meiosis Two [10, 12]. Furthermore, Cdc20p degradation is mediated by APC/CAma1 [12]. In this study, nosotros present evidence that Ama1p down regulation occurs via ubiquitin-mediated deposition directed past APC/CCdc20. Taken together, these results indicate that the prison cell has solved the trouble of APC/C inactivation in a linear differentiation pathway by evolving a common degradation system for the activators.

Results

Cdc20p activates the APC/C to mediate Ama1p degradation

We take previously reported that Ama1p levels are reduced as cells complete the second meiotic division [10]. As APC/C activators have been reported to exist down-regulated by APC/C mediated proteolysis during mitotic and meiotic cell divisions (reviewed in [7, eight]), we first asked if the reduction in Ama1p levels was APC/C dependent. The meiotic levels of Ama1p-T7 [12] were monitored in a strain harboring a temperature sensitive allele of CDC16 (cdc16-1), an essential component of the APC/C [31] that is required for meiosis [x]. To inactivate Cdc16-1p, the cells were switched to the restrictive temperature (34.five°C) 4.5 h after meiotic entry as previously described [8, 10, 32]). As a control, Ama1p degradation was also examined in identically treated wild-blazon cells. Immunoblot analysis revealed that Ama1p-T7 levels remained elevated in the cdc16-1 strain compared to wild type (Effigy 1A, quantitated in Figure 1B). Similar results were obtained when these experiments were repeated in a cdc20-1 strain (Effigy 1A). Furthermore, these results are consequent with those obtained when Ama1p levels were monitored in a strain where Cdc20p was inactivated during meiosis by placing it under the control of CLB2 promoter [33]. Taken together, these results indicate that APC/CCdc20 is required for the down regulation of Ama1p-T7 in meiosis.

Figure one
figure 1

APC/C Cdc20 is required for Ama1 degradation during meiosis. A: Wild-type (RSY335), cdc20-i (RSY809) and cdc16-1 strains (RSY954) harboring Ama1p-T7 (pKC3036) were induced to enter the meiosis and timepoints taken as indicated. Immunoblot analysis of immunoprecipitated protein extracts was conducted to detect Ama1p. Immunoblot assay of Tub1p was used as a loading control. MI and MII indicate the approximate times of meiosis I (MI) and meiosis II (MII) as determined by DAPI analysis. All the strains were grown at 23°C and switched to 34.5°C (restrictive temperature for both cdc20-one and cdc16-ane strains) afterwards 4.5 h at 23°C in SPM. B: Quantitation of Ama1p-T7 from the experiments conducted in A. C: The levels of Ama1p-T7 were monitored in a cdc20-one strain equally in Console A except that the cells were switched to the restrictive temperature 15 h later on transfer to SPM. This panel also contains assay of Ama1p-T7 stability at both temperatures, 30 h after inbound sporulation. D: As in Panel A except that the wild type (RSY335) and cdc20-ane (RSY809) cultures harbored either Clb5p-3HA (pKC440) or Clb1p-9HA (pKC427) expression plasmids.

Full size prototype

A caveat to this interpretation is that Ama1p-T7 stabilization in the cdc20-1 mutant is an indirect result of the metaphase I arrest associated with this mutation [32]. To address this issue, ii approaches were taken. Beginning, we examined Ama1p stability in a cdc20-1 mutant shifted to the restrictive temperature following meiosis 2 (15 h timepoint). These results show that Ama1p remains stable in the cdc20-one strain at restrictive temperature even following 30 h in SPM (Figure 1C). To confirm that the cdc20-1 cells had completed the meiotic divisions by this timepoint, the transcription profiles of meiosis-specific genes were monitored using Northern blot analysis. Past 15 h in SPM, maximal transcriptional accumulation of SPS4 was observed (Additional file ane) which is an indicator that the meiotic divisions are completing [34]. Similarly, SPS100 mRNA induction, which correlates with spore wall formation [35], occurs xviii h after meiotic entry.

For the second approach, we analyzed the meiotic degradation of Clb5p, a known substrate of APC/CCdc20 [11]. Clb5p-HA levels were followed by immunoblot assay in wild type and cdc20-one cultures using the same temperature shift protocol as described in panel A. The results evidence that, compared to wild-type cells, Clb5p was stabilized following Cdc20p-ane inactivation (Effigy 1D). In contrast, Clb1p, a known substrate of APC/CAma1 [10], is destroyed in cdc20-i cells using the same weather condition (Figure 1D). The slower induction kinetics observed for both cyclins is due to the fact that expression of early-eye, middle gene mRNAs is significantly reduced as well as delayed in this strain background [32]. Taken together, these results support a model that APC/CCdc20 mediates the degradation of Ama1p as cells complete the meiosis and begin spore morphogenesis.

Cdh1p is not required to mediate the degradation of Ama1p during meiosis

To determine whether Cdh1p plays a part in Ama1p proteolysis during meiosis, Ama1p protein levels were monitored in cdh1∆ cells during meiosis. The results prove that cdh1∆ cells both progress through meiosis (Additional file 2: Figure S2A, S2B and S2C) and degrade Ama1p with the same kinetics every bit wild type (Boosted file 2: Figure S2D and see Tan et al. [12] for Northern analysis). Interestingly, dissection of the resulting cdh1∆ tetrads revealed that, dissimilar to previously published results [16], cdh1∆ spores exhibit a meaning reduction in their power to form colonies (Additional file 2: Effigy S2E). These results bespeak that Cdh1p does not command Ama1p stability simply does play a role in promoting spore viability.

Ama1p contains functional degradation signals

Ama1p contains two motifs, the devastation box (Db) and GxEN, that are recognized by APC/CCdc20 (reviewed in [36]), come across Figure 2A). To determine if these sequences are required for Ama1p-T7 deposition, wild-blazon cells expressing either Ama1pDb1∆-T7 or Ama1pGxEN-T7 mutant proteins were induced to enter meiosis and their degradation profiles monitored by immunoblot analysis. These studies revealed no difference in decay kinetics for the single mutant derivatives compared to wild type (Figure 2B) indicating that individually the Db1 or GxEN motifs are not essential for Ama1p degradation. We have recently shown that the APC/CAma1 mediates Cdc20p deposition through more than i degron [12]. To determine if Cdc20p too recognizes multiple Ama1p degrons, wild-blazon cells expressing a double Db1 and GxEN AMA1 derivative were examined as merely described. The results (Figure 2B, quantified in Figure 2C) testify that combining the GxEN and Db1 mutations protected Ama1p-T7 from degradation similar to that observed in cdc16-1 cells (compare to Figure 1A). These results indicate that either Db1 or GxEN is sufficient to target Ama1p for degradation. No difference in the charge per unit of meiotic progression (Figure second) or spore viability (Figure 2E) was noted indicating that stabilizing Ama1p did not take an adverse effect on the process.

Figure 2
figure 2

Identification of Ama1p degrons. A: Location of conserved APC/C degrons in Ama1p. The consensus sequences of destruction box and GXEN motifs are in bold confront. The mutations described in the text are indicated below the consensus sequences. B: Both Db1 and GxEN degrons mediate Ama1p degradation during meiosis. Wild-type cells (RSY335) harboring plasmids expressing Ama1p-T7 or mutants as indicated were induced to enter meiosis and samples taken for immunoprecipitation and immunoblot assay at the timepoints indicated. Tub1p levels were used as a loading control. C: Quantitation of the degradation kinetics of wild-type Ama1p-T7 and the Db1-GxEN double mutant obtained in Panel B. The hateful ± s.e.m. is shown for each timepoint (n=iii independent experiments). D: The percentage of tetra-nucleated cells during a meiotic timecourse in ama1∆ cells (RSY562) expressing either wild-type Ama1p (squares) or the DB1/GxEN double mutant (circles) plasmids. Due east: Fluorescence microscopy (1000X magnification) and Nomarski optics (Nom.) of DAPI Db1/GxEN expression plasmids. The percent viability of dissected spores (n=40, WT normalized to 100%) is given below.

Full size image

Ama1p is a substrate of APC/CCdc20 in vitro

To farther ostend that APC/CCdc20 mediates the degradation of Ama1p, in vitro ubiquitylation assays were performed (run across Methods for details). Every bit Ama1p is an activator of the APC/C [x], the assays were performed with an in vitro transcription coupled translation produced 35-S labeled Ama1p derivative deleted for its two APC/C binding domains (C-box and IR motif). These motifs are required for Ama1p role [12] and their mutation reduces its association with the APC/C (meet Figure 3B and C). To ensure that the added Cdc20p is the but activator in the reaction, the APC/C core complex was purified from mitotically dividing cdh1∆ cells. Furthermore, Mnd2p (Apc15p) was not present in the extracts as it inhibits meiotic APC/C activity [33]. As predicted from the in vivo studies, Ama1pCB∆/IR∆ is ubiquitylated by APC/CCdc20 in vitro (Figure 4A, lanes i, 2 and 3 and meet Additional file iii for input), merely also that Cdc20p is required for this event (Figure 4A – lane 12).

Figure iii
figure 3

Ama1p bounden to the APC/C is not required for its deposition. A: ama1∆ strain (RSY562) harboring either Ama1p-T7 (pKC3036) or Ama1pCB∆/IR∆-T7 (pKC3048) expression plasmids were induced to enter meiosis and timepoints taken as indicated. Immunoprecipitation and immunoblot analysis of protein extracts was conducted to notice Ama1p-T7 and Ama1pCB∆/IR∆-T7. Immunoblot analysis of Tub1p was used every bit a loading command. B: Ama1p deleted for the CB and IR regions shows reduced binding to Cdc23p-9myc during meiosis. The Cdc27-9myc expressing strain (KCY328) harboring either the vector command, Ama1p-T7 or mutant versions of Ama1p as indicated were induced to enter meiosis and the cells harvested 12 h following transfer to SPM when both CDC27 and AMA1 are expressed. Immunoprecipitation and immunoblot analysis was conducted to detect the presence of both proteins. The top and centre panels control for protein expression (input). The bottom panel assays co-immunoprecipitation. C: The amino-terminal region (codons ane-200) of Ama1p is sufficient for APC/C clan. The Cdc27-9myc expressing strain RSY1337 harboring either GST (lanes three and 4), GST-Ama1p1-200 (lanes 1, 2 and 5) or GST-Ama1pane-200CB∆ (lane 6) expression plasmids were grown in raffinose/galactose medium to induce the fusion genes. Immunoprecipitation and immunoblot analysis was conducted to detect the presence of both proteins. The top and middle panels control for protein expression (input). The bottom panel assays co-immunoprecipitation. [] represents the no antibody mock immunoprecipitation. The asterisk represents a background band. D: A wild-type strain (RSY750) harboring integrated AMA1-3HA and CDC20-18myc alleles were induced to enter meiosis and timepoints taken as indicated. Immunoblot analysis of immunoprecipitated poly peptide extracts was conducted to observe Ama1p-3HA and Cdc20p-18myc. Immunoblot analysis of Tub1p was used as a loading control. In all experiments, the approximate times of meiosis I (MI) and meiosis II (MII) were determined by DAPI analysis.

Total size image

Effigy 4
figure 4

Ama1p ubiquitylation past APC/C Cdc20 A: in vitro ubiquitylation of Ama1p and mutant derivatives every bit indicated using the APC/C prepared from mnd2∆ cdh1∆ CDC16 ::TAP strain (RSY1381, see Methods for details). In vitro transcription coupled translation produced Cdc20p was added to all extracts except for lane 12. 35S labeled Ama1p harboring the following mutations:- lanes i, 2 and 3 CB∆/IR∆, lanes iv, 5 and 6 CB∆/IR∆/GxEN, lane seven CB∆/IR∆/GxEN/Db1/Db2, lanes viii and ix CB∆/IR∆/Db1 and lanes 10,11 and 12 CB∆/IR∆/GxEN/Db1 was prepared by in vitro transcription coupled translation. B: Doc1p is non required for APC/CCdc20 mediated ubiquitylation of Ama1p. In vitro ubiquitylation assays on Ama1pCB∆/IR∆ using APC/C purified from mnd2∆, cdh1∆ CDC16::TAP (RSY1381, lanes i, 2 and three) or mnd2∆ cdh1∆ doc1∆ CDC16::TAP (RSY1748 lanes iv, 5 and 6). Time after the add-on of Cdc20p to the reactions (minutes at 37°C) is given.

Full size prototype

The in vivo stability assays but described (Figure two) indicated that either Db1 or the GxEN motif is sufficient to induce Ama1p degradation. Consistent with this issue, deletion of either of these motifs in the Ama1pCB/IR mutant still immune ubiquitylation to occur (Figure 4A, lanes iv-6 for GxEN, eight and nine for Db1). However, Ama1p mutated for both Db1 and GxEN was still ubiquitylated in vitro by APC/CCdc20 (Figure 4A, lanes 10 and 11). This upshot was unexpected as this mutant is not targeted for degradation in vivo (Figure 2B). These results led united states of america to test if the second destruction box degron (Db2) on Ama1p tin can mediate Cdc20p-dependent in vitro ubiquitylation. This was indeed the case as the mutation of Db2, in add-on to Db1 and GxEN, rendered Ama1p resistant to APC/CCdc20-dependent ubiquitylation (Figure 4A, lane7). Taken together, these results reveal that Cdc20p tin recognize degrons Db1, Db2 and GxEN using in vitro assays. However, Db2 is non recognized past Cdc20p as a degron in vivo during meiosis.

The APC/C core component Doc1p forms office of the bipartite degron receptor in yeast [19, 25, 30]. Therefore, we addressed whether Doc1p is required for APC/CCd20 mediated ubiquitylation of Ama1p. The ubiquitylation assays were repeated using Ama1pC-Box∆/IR∆ equally the substrate and APC/C was prepared from cdh1∆ mnd2∆ doc1∆ cells. The results prove a slight qualitative reduction in Ama1pC-Box∆/IR∆ ubiquitylation when the APC/C was prepared from cdh1∆ mnd2∆ doc1∆ extracts compared to those prepared from a cdh1∆ mnd2∆ strain (Figure 4B, compare lane iii to 6). These results advise that Doc1p is dispensable for Ama1p ubiquitylation in vitro.

Ama1p association with the APC/C through its C-box and IR motif is not required for its deposition

Significant structural assay of the APC/C and its substrates has found two distinct locations within the cavity of the core APC/C complex that are occupied past the activator protein and the substrate. Our findings that Ama1p is both an activator and a substrate of the APC/C raised the question of its location within the APC/C crenel earlier it was destroyed. To address this question, nosotros took advantage of the observation that the conserved APC/C binding domains of Ama1p (C-box and IR motif) are required for APC/CAma1 function and normal clan with the APC/C [12]. Therefore, we reasoned that if Ama1p was destroyed while in its activator binding pocket, so disruption of this interaction should protect the protein from degradation. Immunoblot blot analysis of ama1∆ cells harboring either wild-type Ama1p or Ama1pCB∆/IR∆-T7 during meiosis revealed no differences in the kinetic profile of Ama1p accumulation and degradation (Figure 3A). These results point that Ama1p clan to the APC/C via the CB and IR motifs is non a pre-requisite for its degradation. These results also advise that the majority of Ama1p degradation is not mediated by auto-ubiquitylation as Ama1pCB∆/IR∆-T7 is withal degraded in the absence of a functional copy of Ama1p.

To further address this question, co-immunoprecipitation performed assays were performed between Cdc27p-9myc and either Ama1p, Ama1pCB∆-T7, Ama1pIR∆-T7, or Ama1pCB∆/IR∆-T7. The results showed that Ama1pCB∆-T7 and Ama1pCB∆/IR∆-T7, which complemented an ama1∆ allele with eleven and <0.5% sporulation efficiency, respectively [12], exhibited reduced Cdc27p-9myc binding (Figure 3B). Conversely, Ama1pIR∆-T7, which exhibited only slight reduction in activity [12], binds Cdc27p-9myc with similar affinity every bit wild-type Ama1p. These results were somewhat unexpected as deleting the IR and Cbox motifs in Cdh1p eliminates its ability to demark the APC/C [37]. In improver, these results suggest the presence of boosted APC/C binding motif(s) in Ama1p. Consistent with this possibility, nosotros establish that a GST-Ama1p fusion construct containing the divergent amino tertiary of Ama1p (codons i-200) [12], can co-immunoprecipitate with Cdc27p-9myc (Figure 3C) whereas GST alone cannot (lanes 3 and 4). Again, we only observe a slight reduction in Cdc27p-9myc association when a GST-Ama1p1-200CB∆ fusion construct (Figure 3C, lane half-dozen). These results bespeak that the amino-terminal region of Ama1p is sufficient for APC/C clan and contains an uncharacterized APC/C binding motif(s).

Cdc20p and Ama1p are degraded with the same kinetics during meiosis

We have previously demonstrated that APC/CAma1 directs the deposition of meiotic Cdc20p [12]. Our results here point that in a reciprocal fashion APC/CCdc20 also mediates the deposition of Ama1p equally cells exit meiosis II. If Ama1p and Cdc20p are required for each other's degradation, one prediction of this model is that their deposition kinetics should be similar. To exam this hypothesis, a strain was constructed harboring integrated alleles of CDC20- 18myc and AMA1-3HA under the control of their own promoters. Our previous studies plant that Ama1p-3HA is both functional and has the aforementioned deposition kinetics every bit Ama1p-T7 [10]. A meiotic timecourse was conducted and Cdc20p-18myc and Ama1p-3HA expression profiles were adamant past immunoblot absorb assay. These studies revealed that the accumulation and subsequent degradation of both proteins were remarkably like (Effigy 3D). These results are consistent with the model that Ama1p and Cdc20p simultaneously mediate each other'due south degradation, thus terminating APC/C activeness every bit the cells consummate meiosis and form quiescent spores.

Conclusions

The APC/C ubiquitin ligase is required for the meiotic nuclear divisions in yeast. Previous studies have found that the ii APC/C activators in meiosis, Ama1p and Cdc20p, are down regulated as cells complete meiosis 2. Cdc20p is targeted for degradation past APC/CAma1 [12]. In this study, we demonstrate that the reverse is true in that APC/CCdc20 is required for Ama1p degradation. Using a combination of stability assays and in vitro ubiquitylation experiments, we show that Cdc20p, but not Cdh1p, targets Ama1p through either ane of two degrons, Db1 and GxEN. Nosotros also provide testify to support a model in which degradation of Ama1p does not occur past car-ubiquitylation equally the non-functional Ama1pCB∆/IR∆ mutant is all the same degraded with wild-type kinetics in ama1∆ cells. Finally, we bear witness that the deposition of Ama1p and Cdc20p at MII exit occurs with like kinetics. Taken together, these results suggest a model in which the mutually dependent deposition of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic evolution in yeast.

Agreement how the APC/C is regulated during both mitotic and meiotic divisions is of import equally unscheduled APC/C activity can lead to mis-segregated chromosomes and aneuploid gametes. Many studies have been devoted dissecting the precise mechanisms by which the APC/C is both activated and inactivated in mitotic cells (reviewed in [5]). These studies revealed that the complete inactivation of the APC/C late in G1 is driven past inhibition of Cdc20p and Cdh1p. This system not only resets the APC/C clock, which is critical for maintaining ploidy as it ensures that the pre-replication complex is assembled prior to South phase (reviewed in [36]). Cdh1p inactivation is achieved by phosphorylation (reviewed in [vii]). However, Cdc20p regulation is more complex. Initially, it was shown that Cdc20p is inactivated past transcriptional oscillation and turnover past APC/CCdh1 (reviewed in [4]). However, recently it was shown that APC/CCdh1 only partially contributes to Cdc20p deposition during anaphase [38]. Instead, Cdc20p degradation is predominantly mediated by an automobile-ubiquitylation upshot [6, 39]. Ama1p degradation does not seem to accept the same grade as the not-functional CB∆/IR∆ is however degraded in ama1∆ cells (Figure 4A).

Fifty-fifty less is known nigh how the APC/C is inactivated as cells exit meiosis Two. This is an important question as APC/C inactivation is important for normal embryonic development in Drosophila [40]. Similarly, we find that the two APC/C activators are degraded late in meiotic development. However, nosotros discover no significant effect on meiosis Ii fidelity or overall spore viability when either Cdc20p or Ama1p degradation is inhibited ([12] and Figure 2). These observations suggest that either APC/C inactivation is not required for the normal execution of meiosis and spore formation or that this ubiquitin ligase is disabled by redundant systems. In support of the latter possibility, several mechanisms are known to control APC/C function including inhibitory phosphorylation [41–44], APC/C specific inhibitors [45–52], or removal of the activator from the APC/C complex [53]. The roles these mechanisms play as cells go out the meiotic program are not well understood. However, in Xenopus and Southward. pombe, inhibitors of meiotic Cdc20p take been identified [54, 55].

Model for substrate recognition by APC/C activators

Extensive studies accept been devoted to understanding the molecular mechanisms of APC/C activator binding and substrate recognition (reviewed in [five]). Currently, two not-mutually sectional models take been proposed. In the bi-partite model (outlined in model A, Effigy 5), the substrate binds to both the activator and to Doc1p in the inner crenel of the APC/C. This dual association increases the affinity of the substrate enzyme circuitous [19, 24, 25, 30]. All the same, Doc1p it is non essential for substrate binding in yeast [56] and its contribution to meiosis is not well documented. In the second model, coined the allosteric model, binding of the activators to the APC/C induces a conformational alter which leads to substrate recognition [57]. Currently, the bipartite model is favored but the two models can co-be as the bi-partite model can still accommodate activator association promoting conformational changes.

Effigy five
figure 5

Possible mechanisms for mutually dependent degradation of Cdc20p and Ama1p. A : Generic APC/C model derived from genetic, biochemical and structural data (adjusted from models presented in [19, 24, 58, 59]); both the activator (green) and the substrate (cherry) are located in the inner cavity of the multi-subunit complex. The substrate is represented as binding between the interface of the activator (via D-box or GxEN) and Doc1p (purple, via D-box [30]). The "platform" (Apc1p, Apc4p and Apc5p) and Apc2p are shown in blueish and the "arc lamp" (Cdc16p, Cdc23p and Cdc27p) in calorie-free brown. The activator is connected to the arc lamp (via Cdc27p) and to the platform (via Apc2p) by its IR and C-box motifs respectively [67]. Doc1p is also connected to Cdc27p via its IR motif and to Apc2p (reviewed in [3, 5]). E2 shuttles into the complex during the course of a polyubiquitylation reaction. B: Trans-model. Ama1p and Cdc20p are destroyed when they are released from the activator bounden position and movement into the substrate position. C: Cis-model. Ama1p remains in the activator position and is destroyed by auto-ubiquitylation. D: Cis-dimer model. Ama1p and Cdc20p remain in the activator position. They are destroyed when they come up in contact with some other APC/C subunit bearing the reciprocal activator.

Full size paradigm

That being said, how does Ama1p fit into these models when it becomes a substrate of the APC/C? Recently, work by Foe et al. [6] has shed some lite on this question. This group demonstrated that the majority of the late mitotic turnover of Cdc20p occurs while Cdc20p is jump as an activator and is driven by auto-ubiquitylation (encounter model in Figure 5C, cis-model). Consistent with this model, Cdc20pIR∆ mutants show increased steady state levels and reduced motorcar-ubiquitylation [three, 6]. In contrast, we present evidence that Ama1p deposition is independent of APC/C binding via the CB and/or IR motifs (see Figures 4 and 3A). Every bit the CB and IR motifs associate with Cdc27p/Cdc23p and Apc2p, respectively [3], our data support a model (outlined in Figure 5B, trans-model) in which Ama1p disassociates from Cdc27/23 and Apc2 before information technology is recognized as a substrate past APC/CCdc20. Thus, the residue clan that we observed betwixt Cdc27p and Ama1pCB∆/IR∆ (Figure 3B and C) could exist due to Ama1p associating with the APC/C in the substrate location. This suggests a model in which C-box and IR motifs ballast Ama1p in the activator position just in their absence, Ama1p switches into the substrate position binding the APC/C via as nevertheless uncharacterized motifs. The machinery that triggers this disassociation remains unknown merely recently it has been shown that phosphorylation of Cdc20p prevents its CB-dependent activation of the APC/C in Xenopus egg extracts [44]. Lastly a "cis-dimer" model (Effigy 5D) where Ama1p remains in the activator position and is degraded when an APC/CCdc20 complex forms a dimer partner is also possible. This model is non favored equally although yeast APC/C be every bit dimers, recent work has shown that the monomers associate along the backbone of the "arc lamp" thus positioning the substrate binding sites in opposite directions [19, sixty].

Finally, the observation that Cdc20p and Ama1p both regulate each other leads to the mechanistic question of which poly peptide is the final one to exist degraded. Analysis of both proteins nether the command of their own promoters in a single meiotic timecourse experiment showed that they were down regulated at the same fourth dimension. These results suggest that it may not exist critical every bit to which activated APC/C molecule is the concluding one. To conclude, these data presented hither allow us to advise a model of how APC/C activators are recognized every bit substrates of the APC/C during meiosis. It remains to be seen if this model is conserved during gametogenesis in other systems.

Methods

Yeast strains and plasmids

The strains used in this study (Table 1) are isogenic to RSY335 [61] and are derived from an SK1 groundwork [62]. The only exception to this is RSY1337 that is isogenic a W303a-related strain RSY10 [63]. The Cdc27-9myc::LEU2 strains (KCY328 and RSY1337) were made by inserting CDC27-9myc tagged allele (P. Hieter) into RSY335 and RSY10 respectively. The mnd2∆:: KANMX cdh1∆::LEU2 CDC16-TAP strain (KCY1381) was made as follows. First, the TAP cassette was inserted into the carboxyl terminus of CDC16 by recombining PCR products from pFA6a-TAP-kanMX6 (D. Barford) to create KCY456. Next, the mnd2∆:: KANMX haploid (KCY419) was created in the opposite mating type using the factor disruption [64]. These two haploids were then mated and an mnd2∆::KAN CDC16::TAP:: KANMX haploid (RSY1248) spore clone was identified that showed 2:2 distribution of the KANMX allele following tetrad assay. CDH1 was deleted from RSY1248 using pWS176 (West. Seufert) to create RSY1381. Finally DOC1 was deleted from this strain using standard gene disruption techniques [64] to create RSY1748. The temperature-sensitive cdc20-1 strain (RSY809) has been previously described [32] . The temperature-sensitive cdc16-1 strain RSY954 was made by back crossing H20c1a5 [10] into the RSY335 strain background 8 times. The strain harboring integrated epitope-tagged alleles of both AMA1 and CDC20 (RSY750) was made by using integrating plasmids containing functional AMA1-3HA [10] and CDC20-18myc (from Westward. Zachariae), respectively. Tables ii and 3 list the oligonucleotides and plasmids used in this written report, respectively. Details of plasmid constructions are available on request. In brief, all the AMA1-T7 tagged plasmids were derived from pKC3036 [12]. The Ama1p expressing plasmids used for ubiquitylation assays were derived from pME67 (D. Morgan). The Cdc20p plasmid used for ubiquitylation assays was pME41 (D. Morgan). The CLB5-3HA plasmid (pKC440) was made by cloning an Xho1-Cla one fragment containing Clb5-3HA (from C. Wittenberg) under the control of its own promotor and terminator into Ycplac222. The Clb1-9HA plasmid was made by first cloning a Pst 1-Pst i fragment from a CLB1/CLB6 contig (from C. Wittenburg) into pRS315 and so inserting 9 repeats of the HA epitope merely upstream of the stop codon to create pKC427. The galactose inducible GST-Ama11-200 fusion construct (pKC3113) has been previously described [12]. In brief, AMA1 was introduced into pEG[KT], which contains GST under the control of the galactose promotor (a gift from M. Solomon). Site directed mutagenesis was used to delete the C-box in this construct to make pKC3071. All mutations were introduced using the Quikchange Site-directed Mutagenesis (SDM) Kit (Stratagene) according to the manufacturer's protocol. All introduced mutations were verified past Dna sequencing (MWG/Operon).

Table 1 Yeast strains used in this written report

Total size table

Table two Oligonucleotides used in this study with their accompanying mutation identified

Full size tabular array

Table 3 Plasmids used in this report

Full size table

Meiotic and mitotic timecourse experiments

Growth and sporulation conditions were achieved as previously described [63]. To permit cdc20-ane and cdc16-1 cultures to exit mitosis and enter the meiotic plan, these cells were maintained at 23°C following transfer to sporulation medium for the amount of time indicated in the text earlier switching to the restrictive temperature (water bath). Quantitation of meiosis I and II was achieved past analyzing four', half-dozen-diamidino-2-phenylindole (DAPI) stained cells every bit described [68]. A Nikon E800 fluorescence microscope was used for all experiments at a concluding magnification of 1000X. At least 200 cells were counted per timepoint. For the experiments using the galactose inducible GST expression constructs (Figure 4C), cells were grown to 1 × x7 cells/ml in ii% raffinose, 2% galactose medium as previously described [69].

Northern blot analysis, protein extract preparation, co-immunoprecipitation and Immunoblot analysis

Northern blot analysis was executed as previously described [32]. Poly peptide extracts for co-immunoprecipitation and Western blot analyses (referred to as Immunoblot in text) were prepared as described [12]. Immunoblot analysis and co-immunoprecipitation experiments were conducted with 100 μg and 1 mg of soluble protein, respectively. Immunoblot signals were detected using goat anti-mouse secondary antibodies conjugated to alkaline phosphatase (Sigma) and the CDP-Star chemiluminescence kit (Tropix, Bedford, MA). Quantitation of Ama1p immunoblot signals from the mem brane was performed with an Epitome Station 4000R (Kodak Inc.) using Molecular Imaging Software (4.0.5) and standardized to tubulin. For all comparative immunoblot analyses, the membranes were treated with the same probe at the same time and the resulting signals were adult to the aforementioned extent.

In vitro ubiquitylation assays

The in vitro ubiquitylation assays were performed as previously described [32, 70]. In brief, the APC/C complex was purified from yeast extracts utilizing tandem analogousness purification (TAP) tagged Cdc16p, a core component of this ubiquitin ligase. The ligase was incubated with E. coli produced ubiquitin conjugating enzyme (made from His6-Ubc4p (from Yard. Solomon) and in vitro transcription/translation produced Cdc20p. The Ama1p substrates were synthesized by in vitro transcription/translation (Promega) but in the presence of 35S-methionine. Equally previously described [seventy], one μl of the substrate was used per reaction (see Additional file 3 for input). The ubiquitylation reactions were conducted for the times indicated with fixed Cdc20p amounts (two.five μl). The reactions were stopped past addition of 2X sample buffer and separated by SDS PAGE. The gels were fixed, soaked in Amplify® (Amersham Biosciences), then dried and subjected to autoradiography.

Abbreviations

APC/C:

Anaphase promoting complex

Db1:

Destruction box (degron)

GxEN:

(devastation degron)

CB:

C-box (APC/C binding motif)

IR:

(APC/C binding motif)

MI:

Meiosis I

MII:

Meiosis Two

WT:

Wild-type

SPM:

Sporulation medium.

References

  1. Herskowitz I: Life style of the budding yeast Saccharomyces cerevisiae . Microbiol Rev 1988, 52: 536–553.

    CAS  PubMed  PubMed Central  Google Scholar

  2. Vershon A, Pierce K: Transcriptional regulation of meiosis in yeast. Curr Opin Prison cell Biol 2000, 12: 334–339. x.1016/S0955-0674(00)00104-6

    CAS  Commodity  PubMed  Google Scholar

  3. Thornton BR, Ng TM, Matyskiela ME, Carroll CW, Morgan Practise, Toczyski DP: An architectural map of the anaphase-promoting complex. Genes Dev 2006, 20: 449–460. x.1101/gad.1396906

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  4. Yu H: Cdc20: a WD40 activator for a cell cycle degradation auto. Mol Cell 2007, 27: iii–16. x.1016/j.molcel.2007.06.009

    CAS  Article  PubMed  Google Scholar

  5. Barford D: Structural insights into anaphase-promoting complex function and mechanism. Philos Trans R Soc Lond B Biol Sci 2011, 366: 3605–3624. 10.1098/rstb.2011.0069

    CAS  Article  PubMed  PubMed Central  Google Scholar

  6. Foe IT, Foster SA, Cheung SK, DeLuca SZ, Morgan Exercise, Toczyski DP: Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism. Curr Biol 2011, 21: 1870–1877. 10.1016/j.cub.2011.09.051

    CAS  Article  PubMed  PubMed Fundamental  Google Scholar

  7. Pesin JA, Orr-Weaver TL: Regulation of APC/C activators in mitosis and meiosis. Annu Rev Cell Dev Biol 2008, 24: 475–499. 10.1146/annurev.cellbio.041408.115949

    CAS  Article  PubMed  PubMed Central  Google Scholar

  8. Cooper KF, Strich R: Meiotic control of the APC/C: similarities & differences from mitosis. Cell Div 2011, vi: 16. ten.1186/1747-1028-half dozen-sixteen

    CAS  Article  PubMed  PubMed Central  Google Scholar

  9. Chu S, DeRisi J, Eisen Thousand, Mulholland J, Botstein D, Chocolate-brown PO, Herskowitz I: The transcriptional program of sporulation in budding yeast. Science 1998, 282: 699–705.

    CAS  Article  PubMed  Google Scholar

  10. Cooper KF, Egeland DE, Mallory MJ, Jarnik M, Strich R: Ama1p is a Meiosis-Specific Regulator of the Anaphase Promoting Complex/Cyclosome in yeast. Proc. Natl. Acad. Sci. Us 2000, 97: 14548–14553. 10.1073/pnas.250351297

    CAS  Article  PubMed  PubMed Primal  Google Scholar

  11. Diamond AE, Park JS, Inoue I, Tachikawa H, Neiman AM: The anaphase promoting complex targeting subunit Ama1 links meiotic exit to cytokinesis during sporulation in Saccharomyces cerevisiae. Mol Biol Cell 2009, xx: 134–145. x.1091/mbc.E08-06-0615

    CAS  Article  PubMed  PubMed Central  Google Scholar

  12. Tan GS, Magurno J, Cooper KF: Ama1p-activated anaphase-promoting complex regulates the destruction of Cdc20p during meiosis Ii. Mol Biol Prison cell 2011, 22: 315–326. 10.1091/mbc.E10-04-0360

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  13. Rabitsch KP, Toth A, Galova K, Schleiffer A, Schaffner G, Aigner E, Rupp C, Penkner AM, Moreno-Borchart Air conditioning, Primig M, et al.: A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol 2001, eleven: 1001–1009. 10.1016/S0960-9822(01)00274-3

    CAS  Article  PubMed  Google Scholar

  14. McDonald CM, Cooper KF, Winter E: The Ama1-Directed Anaphase-Promoting Complex Regulates the Smk1 Mitogen-Activated Protein Kinase During Meiosis in Yeast. Genetics 2005, 171: 901–911. x.1534/genetics.105.045567

    CAS  Article  PubMed  PubMed Central  Google Scholar

  15. Coluccio A, Bogengruber E, Conrad MN, Dresser ME, Briza P, Neiman AM: Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell 2004, 3: 1464–1475. x.1128/EC.3.half dozen.1464-1475.2004

    CAS  Commodity  PubMed  PubMed Cardinal  Google Scholar

  16. Kamieniecki RJ, Liu L, Dawson DS: FEAR but not MEN genes are required for exit from meiosis I. Prison cell Wheel 2005, 4: 1093–1098.

    CAS  Article  PubMed  Google Scholar

  17. Glotzer One thousand, Murray AW, Kirschner MW: Cyclin is degraded by the ubiquitin pathway. Nature 1991, 349: 132–138. 10.1038/349132a0

    CAS  Commodity  PubMed  Google Scholar

  18. Pfleger CM, Lee Eastward, Kirschner MW: Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex. Genes Dev 2001, 15: 2396–2407. x.1101/gad.918201

    CAS  Article  PubMed  PubMed Central  Google Scholar

  19. Buschhorn BA, Petzold 1000, Galova Thou, Dube P, Kraft C, Herzog F, Stark H, Peters JM: Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 2011, eighteen: 6–thirteen. 10.1038/nsmb.1979

    CAS  Article  PubMed  PubMed Central  Google Scholar

  20. Barford D: Construction, function and mechanism of the anaphase promoting complex (APC/C). Q Rev Biophys 2011, 44: 153–190. x.1017/S0033583510000259

    CAS  Commodity  PubMed  Google Scholar

  21. Male monarch EM, van der Sar SJ, Hardwick KG: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are disquisitional for the spindle checkpoint. PLoS I 2007, ii: e342. 10.1371/journal.pone.0000342

    Article  PubMed  PubMed Central  CAS  Google Scholar

  22. Bolte M, Dieckhoff P, Krause C, Braus GH, Irniger South: Synergistic inhibition of APC/C by glucose and activated Ras proteins can exist mediated by each of the Tpk1–iii proteins in Saccharomyces cerevisiae. Microbiology 2003, 149: 1205–1216. x.1099/mic.0.26062-0

    CAS  Article  PubMed  Google Scholar

  23. Yamano H, Gannon J, Mahbubani H, Hunt T: Jail cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts. Mol Cell 2004, 13: 137–147. 10.1016/S1097-2765(03)00480-v

    CAS  Article  PubMed  Google Scholar

  24. Da Fonseca PC, Kong EH, Zhang Z, Schreiber A, Williams MA, Morris EP, Barford D: Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. Nature 2011, 470: 274–278. 10.1038/nature09625

    CAS  Article  PubMed  PubMed Central  Google Scholar

  25. Passmore LA, McCormack EA, Au SW, Paul A, Willison KR, Harper JW, Barford D: Doc1 mediates the activeness of the anaphase-promoting circuitous by contributing to substrate recognition. EMBO J 2003, 22: 786–796. 10.1093/emboj/cdg084

    CAS  Article  PubMed  PubMed Central  Google Scholar

  26. Burton JL, Tsakraklides V, Solomon MJ: Assembly of an APC-Cdh1-substrate complex is stimulated past engagement of a devastation box. Mol Prison cell 2005, 18: 533–542. 10.1016/j.molcel.2005.04.022

    CAS  Article  PubMed  Google Scholar

  27. Kraft C, Vodermaier HC, Maurer-Stroh S, Eisenhaber F, Peters JM: The WD40 propeller domain of Cdh1 functions as a destruction box receptor for APC/C substrates. Mol Cell 2005, 18: 543–553. 10.1016/j.molcel.2005.04.023

    CAS  Article  PubMed  Google Scholar

  28. Izawa D, Pines J: How APC/C-Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 2011, 13: 223–233. 10.1038/ncb2165

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  29. Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl K, Peters JM, Huber R, Sondermann P: Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex. Nat Struct Biol 2001, 8: 784–788. 10.1038/nsb0901-784

    CAS  Article  PubMed  Google Scholar

  30. Carroll CW, Enquist-Newman 1000, Morgan Practise: The APC subunit Doc1 promotes recognition of the substrate devastation box. Curr Biol 2005, xv: 11–xviii. 10.1016/j.cub.2004.12.066

    CAS  Article  PubMed  Google Scholar

  31. Lamb JR, Michaud WA, Sikorski RS, Hieter PA: Cdc16p, Cdc23p and Cdc27p form a circuitous essential for mitosis. EMBO J 1994, 13: 4321–4328.

    CAS  PubMed  PubMed Cardinal  Google Scholar

  32. Mallory MJ, Cooper KF, Strich R: Meiosis-specific destruction of the Ume6p repressor past the Cdc20-directed APC/C. Mol Prison cell 2007, 27: 951–961. x.1016/j.molcel.2007.08.019

    CAS  Article  PubMed  PubMed Cardinal  Google Scholar

  33. Oelschlaegel T, Schwickart Yard, Matos J, Bogdanova A, Camasses A, Havlis J, Shevchenko A, Zachariae W: The yeast APC/C subunit Mnd2 prevents premature sis chromatid separation triggered past the meiosis-specific APC/C-Ama1. Jail cell 2005, 120: 773–788. ten.1016/j.jail cell.2005.01.032

    CAS  Article  PubMed  Google Scholar

  34. Hepworth SR, Ebisuzaki LK, Segall J: A xv-base-pair element activates the SPS4 gene midway through sporulation in Saccharomyces cerevisiae. Mol Cell Biol 1995, 15: 3934–3944.

    CAS  PubMed  PubMed Central  Article  Google Scholar

  35. Police DTS, Segall J: The SPS100 gene of Saccharomyces cerevisiae is activated late in the sporulation procedure and contributes to spore wall maturation. Mol Cell Biol 1988, eight: 912–922.

    CAS  PubMed  PubMed Key  Article  Google Scholar

  36. Harper JW, Burton JL, Solomon MJ: The anaphase-promoting complex: information technology'south not just for mitosis any more than. Genes Dev 2002, sixteen: 2179–2206. 10.1101/gad.1013102

    CAS  Article  PubMed  Google Scholar

  37. Schwab M, Neutzner Thousand, Mocker D, Seufert W: Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC. EMBO J 2001, 20: 5165–5175. 10.1093/emboj/20.18.5165

    CAS  Article  PubMed  PubMed Central  Google Scholar

  38. Robbins JA, Cross FR: Regulated degradation of the APC coactivator Cdc20. Cell Div 2010, five: 23. 10.1186/1747-1028-5-23

    Article  PubMed  PubMed Fundamental  CAS  Google Scholar

  39. Foster SA, Morgan Exercise: The APC/C subunit Mnd2/Apc15 promotes Cdc20 autoubiquitination and spindle associates checkpoint inactivation. Mol Cell 2012, 47: 921–932. 10.1016/j.molcel.2012.07.031

    CAS  Commodity  PubMed  PubMed Key  Google Scholar

  40. Pesin JA, Orr-Weaver TL: Developmental role and regulation of cortex, a meiosis-specific anaphase-promoting complex/cyclosome activator. PLoS Genet 2007, 3: e202. 10.1371/journal.pgen.0030202

    Commodity  PubMed  PubMed Central  CAS  Google Scholar

  41. Rudner Advertising, Murray AW: Phosphorylation by cdc28 activates the Cdc20-dependent activeness of the anaphase-promoting complex. J Cell Biol 2000, 149: 1377–1390. x.1083/jcb.149.7.1377

    CAS  Article  PubMed  PubMed Key  Google Scholar

  42. Tang Z, Shu H, Oncel D, Chen Due south, Yu H: Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol Cell 2004, 16: 387–397. 10.1016/j.molcel.2004.09.031

    CAS  Commodity  PubMed  Google Scholar

  43. Chung East, Chen RH: Phosphorylation of Cdc20 is required for its inhibition by the spindle checkpoint. Nat Cell Biol 2003, 5: 748–753. 10.1038/ncb1022

    CAS  Commodity  PubMed  Google Scholar

  44. Labit H, Fujimitsu K, Bayin NS, Takaki T, Gannon J, Yamano H: Dephosphorylation of Cdc20 is required for its C-box-dependent activation of the APC/C. EMBO J 2012, 31: 3351–3362. 10.1038/emboj.2012.168

    CAS  Article  PubMed  PubMed Cardinal  Google Scholar

  45. Reimann JD, Freed Eastward, Hsu JY, Kramer ER, Peters JM, Jackson PK: Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 2001, 105: 645–655. 10.1016/S0092-8674(01)00361-0

    CAS  Article  PubMed  Google Scholar

  46. Martinez JS, Jeong DE, Choi Due east, Billings BM, Hall MC: Acm1 is a negative regulator of the CDH1-dependent anaphase-promoting complex/cyclosome in budding yeast. Mol Cell Biol 2006, 26: 9162–9176. 10.1128/MCB.00603-06

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  47. Choi Due east, Dial JM, Jeong DE, Hall MC: Unique D box and KEN box sequences limit ubiquitination of Acm1 and promote pseudosubstrate inhibition of the anaphase-promoting complex. J Biol Chem 2008, 283: 23701–23710. 10.1074/jbc.M803695200

    CAS  Article  PubMed  PubMed Cardinal  Google Scholar

  48. Ostapenko D, Burton JL, Wang R, Solomon MJ: Pseudosubstrate inhibition of the anaphase-promoting complex by Acm1: regulation by proteolysis and Cdc28 phosphorylation. Mol Cell Biol 2008, 28: 4653–4664. 10.1128/MCB.00055-08

    CAS  Article  PubMed  PubMed Primal  Google Scholar

  49. Enquist-Newman One thousand, Sullivan Grand, Morgan Exercise: Modulation of the mitotic regulatory network by APC-dependent destruction of the Cdh1 inhibitor Acm1. Mol Cell 2008, 30: 437–446. x.1016/j.molcel.2008.04.004

    CAS  Article  PubMed  PubMed Fundamental  Google Scholar

  50. Burton JL, Solomon MJ: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint. Genes Dev 2007, 21: 655–667. 10.1101/gad.1511107

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  51. Sczaniecka G, Feoktistova A, May KM, Chen JS, Blyth J, Gould KL, Hardwick KG: The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem 2008, 283: 23039–23047. ten.1074/jbc.M803594200

    CAS  Article  PubMed  PubMed Central  Google Scholar

  52. Lara-Gonzalez P, Scott MI, Diez Yard, Sen O, Taylor SS: BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner. J Cell Sci 2011, 124: 4332–4345. ten.1242/jcs.094763

    CAS  Article  PubMed  PubMed Central  Google Scholar

  53. Jaquenoud M, Van Drogen F, Peter M: Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1). EMBO J 2002, 21: 6515–6526. 10.1093/emboj/cdf634

    CAS  Commodity  PubMed  PubMed Fundamental  Google Scholar

  54. Schmidt A, Duncan PI, Rauh NR, Sauer Thou, Fry AM, Nigg EA, Mayer TU: Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity. Genes Dev 2005, xix: 502–513. 10.1101/gad.320705

    CAS  Article  PubMed  PubMed Primal  Google Scholar

  55. Kimata Y, Kitamura K, Fenner Northward, Yamano H: Mes1 controls the meiosis I to meiosis 2 transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast. Mol Biol Cell 2011, 22: 1486–1494. 10.1091/mbc.E10-09-0774

    CAS  Article  PubMed  PubMed Fundamental  Google Scholar

  56. Hwang LH, Murray AW: A novel yeast screen for mitotic abort mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Jail cell 1997, eight: 1877–1887. 10.1091/mbc.8.10.1877

    CAS  Article  PubMed  PubMed Central  Google Scholar

  57. Passmore LA, Barford D: Coactivator functions in a stoichiometric circuitous with anaphase-promoting circuitous/cyclosome to mediate substrate recognition. EMBO Rep 2005, 6: 873–878. 10.1038/sj.embor.7400482

    CAS  Commodity  PubMed  PubMed Fundamental  Google Scholar

  58. Matyskiela ME, Morgan Do: Assay of activator-bounden sites on the APC/C supports a cooperative substrate-binding mechanism. Mol Cell 2009, 34: 68–eighty. 10.1016/j.molcel.2009.02.027

    CAS  Commodity  PubMed  PubMed Primal  Google Scholar

  59. Schreiber A, Stengel F, Zhang Z, Enchev RI, Kong EH, Morris EP, Robinson CV, Da Fonseca PC, Barford D: Structural basis for the subunit assembly of the anaphase-promoting complex. Nature 2011, 470: 227–232. ten.1038/nature09756

    CAS  Article  PubMed  Google Scholar

  60. Passmore LA, Booth CR, Venien-Bryan C, Ludtke SJ, Fioretto C, Johnson LN, Chiu West, Barford D: Structural assay of the anaphase-promoting circuitous reveals multiple active sites and insights into polyubiquitylation. Mol Jail cell 2005, 20: 855–866. ten.1016/j.molcel.2005.11.003

    CAS  Article  PubMed  Google Scholar

  61. Cooper KF, Mallory MJ, Strich R: Oxidative stress-induced destruction of the yeast C-blazon cyclin Ume3p requires the Phosphatidylinositol-specific phospholipase C and the 26S proteasome. Mol Cell Biol 1999, nineteen: 3338–3348.

    CAS  PubMed  PubMed Cardinal  Article  Google Scholar

  62. Cooper KF, Mallory MJ, Guacci 5, Lowe K, Strich R: Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae. Genetics 2009, 181: 65–79.

    CAS  Commodity  PubMed  PubMed Key  Google Scholar

  63. Cooper KF, Mallory MJ, Smith JS, Strich R: Stress and developmental regulation of the yeast C-type cyclin UME3 ( SRB11 / SSN8 ). EMBO J 1997, 16: 4665–4675. 10.1093/emboj/sixteen.15.4665

    CAS  Commodity  PubMed  PubMed Key  Google Scholar

  64. Longtine MS, McKenzie AR, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR: Boosted modules for versatile and economical PCR-based factor deletion and modification. Saccharomyces cerevisiae 1998, Yeast fourteen: 953–961.

    Google Scholar

  65. Shirayama M, Zachariae W, Ciosk R, Nasmyth K: The Polo-like kinase Cdc5p and the WD-repeat poly peptide Cdc20p/fizzy are regulators and substrates of the anaphase promoting circuitous in Saccharomyces cerevisiae . EMBO J 1998, 17: 1336–1349. x.1093/emboj/17.5.1336

    CAS  Article  PubMed  PubMed Central  Google Scholar

  66. Schwab Chiliad, Neutzer Thousand, Mocker D, Seufert Westward: Yeast Hct1 recognises the mitotic Clb2 and other substrates of the ubiquitin ligase APC. EMBO 2001, xx: 5165–5175. 10.1093/emboj/20.xviii.5165

    CAS  Article  Google Scholar

  67. Burton JL, Solomon MJ: D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct bounden to Cdc20p and Cdh1p. Genes Dev 2001, 15: 2381–2395. 10.1101/gad.917901

    CAS  Commodity  PubMed  PubMed Central  Google Scholar

  68. Cooper KF, Strich R: Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p is required for efficient induction and execution of meiotic evolution. Eukaryot Cell 2002, 1: 66–74. 10.1128/EC.01.ane.66-74.2002

    CAS  Article  PubMed  PubMed Central  Google Scholar

  69. Prinz S, Hwang ES, Visintin R, Amon A: The regulation of Cdc20 proteolysis reveals a part for APC components Cdc23 and Cdc27 during S phase and early mitosis. Curr Biol 1998, eight: 750–760. ten.1016/S0960-9822(98)70298-two

    CAS  Commodity  PubMed  Google Scholar

  70. Passmore LA, McCormack EA, Au SWN, Paul A, Willison KR, Harper JW, Barford D: Doc1 mediates the activity of the anaphase promoting complex by contributing to substrate recognition. EMBO 2003, 22: 786–796. 10.1093/emboj/cdg084

    CAS  Article  Google Scholar

Download references

Acknowledgements

We thank D. Bradford, P. Hieter, D. Morgan, W. Seufert, K. Solomon, C. Wittenberg and Due west. Zachariae for plasmids. This piece of work was supported by ACS grant # CCG106162 to K. F. C. and past Public Health Service grant #'s CA-099003 and GM086788 from the National Institutes of Health, United states of americaA. to R. S.

Writer information

Author notes

  1. Michael J Mallory

    Present address: Department of Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, 3700 Hamilton Walk, 19104, USA

  2. Grace S Tan

    Nowadays address: Partitioning of Cancer Pathobiology, The Children's Hospital of Philadelphia, Colket Translational Enquiry Building - RM 4300, 3500 Civic Center Blvd., Philadelphia, PA, 19104, U.s.a.

Affiliations

  1. Department of Pathology and Laboratory Medicine, The Children Hospital of Philadelphia, 3501 Borough Center Boulevard, CTRB RM 4300, Philadelphia, PA, 19104, USA

    Grace S Tan

  2. Department of Molecular Biological science, UMDNJ-SOM, 2 Medical Center Bulldoze, 08084, U.s.

    Grace S Tan, Rebecca Lewandowski, Randy Strich & Katrina F Cooper

Corresponding author

Correspondence to Katrina F Cooper.

Boosted information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

GT performed the experiments outlined in Effigy 1A, B and C, 2 and 3A and B. RL performed the experiments outlined in Effigy four. MM performed the experiment in Figure 1D and 3C. KFC and RS wrote the manuscript. All authors read and approved the final manuscript.

Electronic supplementary cloth

13008_2013_182_MOESM1_ESM.tiff

Additional file ane: Assay of cdc20-1 during meiosis. A: Northern blot analysis of cdc20-1 cells progressing through meiosis at 23°C showing the expression of early on (IME2), early middle (NDT80), eye (SPS4) and belatedly genes (SPS100). ENO1 represents the loading control. (TIFF 474 KB)

13008_2013_182_MOESM2_ESM.tiff

Boosted file 2: Cdh1p is non required to degrade Ama1p during meiosis. A: Fluorescence and Nomarski (Nom.) images (1000X magnification) of DAPI stained wild type (RSY335) and cdh1∆ (RSY777) diploids 24 h after transfer to sporulation medium. B: Rate of appearance of bi- and tetranucleated cells in wild type and cdh1∆ cells afterward entry into the meiotic program. Percentage of cells in the culture executing at least one meiotic partition, presented as a function of time following transfer to sporulation medium. MI, Meiosis I; MII meiosis 2. C: % mono, bi and tetranucleated cells in the full population later on 24 h in sporulation medium. D: cdh1∆ strain (RSY777) harboring Ama1p-T7 (pKC3036) was induced to enter meiosis and timepoints taken as indicated. Immunoblot analysis of immunoprecipitated poly peptide extracts was conducted to find Ama1p-T7. Immunoblot analysis of Tub1p was used equally a loading command. E: Viability of wild type (RSY335) and cdh1∆ (RSY777) tetrad spores. (TIFF 684 KB)

13008_2013_182_MOESM3_ESM.tiff

Boosted file iii: 35 S labeled Ama1p input for ubiquitylation assays. 1 μl of 35S labeled in vitro transcription/translation Ama1p prepared from either pKC3095 (lane one), pKC3122 (lane 2) pKC3148 (lane 3) or pKC3124 (lane iv) or zip Dna control was visualized by autoradiography. (TIFF 297 KB)

Authors' original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed nether the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted utilize, distribution, and reproduction in whatsoever medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Tan, Grand.S., Lewandowski, R., Mallory, Chiliad.J. et al. Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic evolution in yeast. Cell Div 8, 9 (2013). https://doi.org/10.1186/1747-1028-8-9

Download citation

  • Received:

  • Accustomed:

  • Published:

  • DOI : https://doi.org/10.1186/1747-1028-8-ix

Keywords

  • Cdc20p
  • Ama1p
  • Anaphase Promoting Complex
  • Meiosis

leblanchantivane.blogspot.com

Source: https://celldiv.biomedcentral.com/articles/10.1186/1747-1028-8-9

0 Response to "analysis of a cell shows that cdc20 is bound to apc. what else should be true?"

Post a Comment

Subscribe to: Post Comments (Atom)

Iklan Atas Artikel

Iklan Tengah Artikel 1

Iklan Tengah Artikel 2

Iklan Bawah Artikel